Combined detection test for circulating FeLV (Feline Leukemia Virus) antigens, anti-FIV (Feline Immunodeficiency Virus) antibodies and anti-Feline Corona Virus (FCOV) antibodies in cats.
Cat
Diagnostic test
Quick and easy to use, Speed Trio FeLV/FIV/Corona tests provide results for 3 diseases in just 15 minutes.
Regular screening of cats, especially if a risk of exposure or contact exists
Only 3 drops of sample to detect 3 feline viruses
Confirmation when a clinical suspicion exists
Product specifications
Sample
Anticoagulated whole blood, serum or plasma
Target species
Cats
Manipulation time
2 minutes
Time-to-results
15 minutes
Shelf life
Long shelf life of 24 months at room temperature (2°C - 30°C)
Presentation
Box of 6 tests
For veterinary use only.
Speed Trio FeLV/FIV/Corona
Why use Speed Trio FeLV/FIV/Corona ?
FeLV and FIV are responsible for non specific and highly variable symptoms. Moreover, the clinical phase is preceded by an aymptomatic phase of varying duration. This means that biological tests are essential for differential diagnosis. (3) Regular screening of cats to assess their status with regard to retroviruses (FeLV or FIV) is considered the best way of combating these viruses (2). Depending on the region concerned, studies show prevalences of between 1% and 20%.
Testing for soluble FeLV viral antigens enables us to determine the animal's viremic status, even in the event of a low blood viral load (early infection, transient viremia, sequestered infection). However, an antigen test can be negative in case of latent infection.
Detection of anti-FCoV antibodies is useful for guiding preventive measures and can be used for FCoV control in a multi-cat environment. A positive FCoV antibody test indicates that the cat has been infected/exposed to FCoV and has developed antibodies; seroconversion usually occurs within 7 to 28 days post-infection (1). The test does not differentiate between FeCV and the virus responsible for FIP, and does not necessarily indicate active infection.
When to use Speed Trio FeLV/FIV/Corona ?
Speed Trio FeLV/FIV/Corona can be used :
To screen apprently healthy cats with or without known history prior to vaccination, sterilization, breeding or about to be introduced into a multi-cat environment and guide the separation of infected and healthy animals
To confirm or rule-out an exposition to retroviruses or retroviral clinical suspicion
In combination with other biological tests, add diagnostic value and help reduce the hypothesis of feline infectious peritonitis in cases of clinical suspicion.
In combination with other biological tests (PCR, ELISA...), to know the stage of the retroviral disease and its evolution over time (latency or active infection)
Quick guide
For complete instruction, please refer to the product notice.
1. Add 1 drop of sample into each sample well.
2. Add slowly 5 drops of reagent into each sample well.
3. Read the result at 15 minutes.
Speed Trio FeLV/FIV/Corona performances
Reference method
FeLV Viral isolation
FIV Western blot
FCoV IFI*
Sensitivity vs ref
94.7%
96.3%
96.5%
Specificity vs ref
99.2%
98.9%
100%
* Indirect Immunofluorescence
Retroviruses :
(1) HARTMANN K. et coll. Quality of different in-clinic test systems for feline immunodeficiency virus and feline leukaemia virus infection. Journal of Feline Medicine and Surgery, 2007, 9, 439-445
(2) Giselbrecht et al., Prevalence of Different Courses of Feline Leukaemia Virus Infection in Four European Countries, Viruses. 2023 Aug; 15(8): 1718.
(3) Murphy et al., The Late Asymptomatic and Terminal Immunodeficiency Phases in Experimentally FIV-Infected Cats—A Long-Term Study; Viruses. 2023 Aug; 15(8): 1775.
(4) Little, Levy et al;.2020 AAFP Feline Retrovirus Testing and Management Guidelines; J of Fel Med Surg Volume 22, Issue 1, January 2020, Pages 5-30
(5) Buch J., Beall M, o’Connor T. Worldwide clinic-based serologic survey of FIV antibody and FeLV antigen in cats. ACVIM Forum Research Abstract Program, J Vet Intern Med. 2017; 31: 1315
(6) Levy JK, Crawford PC, Tucker SJ; Performance of 4 Point-of-Care Screening Tests for Feline Leukemia Virus and Feline Immunodeficiency Virus; J Vet Intern Med 2017 Mar;31(2):521-526. doi: 10.1111/jvim.14648. Epub 2017 Feb 3.
Coronaviruses :
(1) Tasker S, Addie DD, Egberink H, Hofmann-Lehmann R, Hosie MJ, Truyen U, Belák S, Boucraut-Baralon C, Frymus T, Lloret A, Marsilio F, Pennisi MG, Thiry E, Möstl K, Hartmann K. Feline Infectious Peritonitis: European Advisory Board on Cat Diseases Guidelines. Viruses. 2023 Aug 31;15(9):1847. doi: 10.3390/v15091847. PMID: 37766254; PMCID: PMC10535984.
(3)ADDIE D et al.. Comparison of Speed® feline coronavirus antibody rapid immunomigration tests with inderect immunofluorescence. 2008, Feline Institute Pyrenees.
(4) CACHON T., CHUZEL T. Epidémiologie, pathogénie et symptômes de la PIF. Point Vétérinaire, 2005, 254, 18-21
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