Speed Cortisol gives accurate quantitative results, directly at the patient's bedside in 15 minutes only.
Speed Cortisol enables veterinarians to test for canine hypocorticism or hypercorticism in a single test, thanks to its extended operating range and low sample volume requirement.
Thanks to the Speed Reader technology, Speed Cortisol test kits enable precise and fully quantitative assay, previously validated by a laboratory reference method.
Speed Cortisol makes it possible to detect and monitor disease progression and treatment efficacy during a single visit.
For veterinary use only.
Features
Technical specifications
Sample
50µL serum
Target
Dogs
Handling time
2 minutes
Incubation
5 minutes
Time-to-results
10 minutes
Operating range
10 - 800 nmol/L
Storage
Store at the refrigerator (+2°C to +8°C) until the shelf life indicated on the external packaging and test pouches
Presentation
Box of 6 tests
Why using Speed Cortisol ?
Cortisol is secreted by the adrenal glands under the control of the hypothalamic-pituitary-adrenal axis.
Measuring cortisol in the blood helps to detect and diagnose hyperadrenocorticism (Cushing's syndrome) and hypoadrenocorticism (Addison's disease) in dogs, thanks to an ACTH stimulation test.
When to use Speed Cortisol ?
Speed Cortisol can be used both for screening purposes and as a follow-up to adapt treatment.
Hypercorticism is more common in dogs aged 6 years and over, especially females. The combination of various side effects of excessive cortisol production leads to Cushing's disease.
Hypocorticism is more common in young dogs (under 6 years of age), and in females as well. Mineralocorticoid deficiency induced by adrenal insufficiency leads to Addison's disease.
Speed Cortisol can therefore be used as part of an ACTH stimulation test to detect the presence of hyper/hypocorticism.
Quick guide
Remove tests, reagents and samples from the refrigerator 30 minutes before analysis, so that test materials are at room temperature.
Start a “new test” on the Speed Reader or from Speed Connect and follow the on-screen instructions.
Pour plus de détails, se référer à la notice d'utilisation des tests incluse dans les kits Speed Cortisol.
Recommendations
Only use serum for the sample.
Serum or plasma should be used at room temperature within the hour, and can be stored in a dry tube in the refrigerator, separated from the red blood cell pellet, for 7 days. After that, the sample should be frozen at -20°C.
Cool or freeze samples at room temperature before use (do not heat).
Follow protocol steps and incubation times precisely.
Follow the pipetting method indicated in the test instructions.
Never mix reagents, test cassettes and calibration chips from one batch to another.
Speed Cortisol performances
Validation of Speed Cortisol versus chemiluminescence immuno-enzymatic (CLEIA) method on plasma/serum samples :
Dogs (N=340)
Regression
y=0,95x - 7,649
Correlation
R2=0,927
Speed Cortisol han excellent correlation with the reference CLEIA dosage in dogs.
References
1. Feldman EC, Nelson RW. Canine and Feline Endocrinology and Reproduction. 3rd ed. St. Louis, Missouri: WB Saunders; 2004. pp. 394–439. [Google Scholar]
2. Ian K. Ramsey, Monitoring Canine Hyperadrenocorticism 2023 ACVIM Hybrid Forum FRCVS, Glasgow, Scotland, UK
3. Fletcher J; Trilostane Treatment and Monitoring: Is the ACTH Stimulation Test Gone for Good? WSAVA congress 2019
4.Hauck C, Prevalence and characterization of hypoadrenocorticism in dogs with signs of chronic gastrointestinal disease: A multicenter study;J Vet Intern Med.2020;34:1399–1405.
5.Carotenuto, Cushing’s syndrome—an epidemiological study based on a canine population of 21,281 dogs, Open Vet J. 2019 Jan-Mar; 9(1): 27–32.
6. Schofield I, et al. Frequency and risk factors for naturally occurring Cushing's syndrome in dogs attending UK primary-care practices, Journal of Small Animal Practice • Vol 63 • April 2022
7. Lim et al., Re-evaluation of the low-dose dexamethasone suppression test in dogs J Small Anim Pract. 2023 Jan;64(1):12-20.
