Cultures

Speed Mam Color

Combined bacterial identification and antibiotic susceptibility test for mastitis in cows

Cattle

Diagnostic test

Speed Mam Color is an "all-in-one" culture medium for the bacterial identification and the determination of antibiotic susceptibility profiles for udder infections in cows.

Determination of the sensitivity profile to 14 single or combined antibiotics in 24-48 hours, including critical antibiotics, in order to identify potential resistance directly from milk.
Identification of 8 bacterias frequently involved in clinical mastitis within 48 hours
Innovative microwell cultivation technology enhances the value of veterinary breeding advice and enables rational prescription
Interpretation facilitated by color change of wells and growth indicators
Some selective agar plate may be inoculated directly from the wells for subspecies identification

For veterinary use only.

Product specifications

Sample	Milk
Antibiotics	Amoxicillin+clavulanic acid, Ampicillin+colistin, Cefalexin, Cefoperazone, Cefquinome, Cloxacilline, Ceftiofur, Gentamicin, Marbofloxacin, Penicillin+streptomycin, Spiramycin, Sulfonamid+Trimethoprim, Tetracylcin+Neomycin+Bacitracin, Tylosin
Bacterias	Staphylococcus spp., Streptococcus spp., Streptococcus uberis, Escherichia coli, Enterococcus spp., Enterobacteriaceae, Pseudomonas aeruginosa, Mycoplasma bovis
Target species	Cows
Handlings	7 minutes
Result reading	Antibiogram: 24h at 37°C, identification : 48h at 37°C, Mycoplasma : 7 days at 37°C
Storage	Store in the fridge, away from light (2°C - 8°C)
Prrsentation	Box of 5 galeries

Why use Speed Mam Color ?

Mastitis is the most common and costly disease affecting dairy herds worldwide. With antimicrobial therapy remaining the mainstay of mastitis control programmes, the development of antibiotic resistance is an issue of great concern.

International pressure to limit antimicrobial therapy in animals and reduce the risk of resistant bacteria continues to grow.

Good mastitis management therefore requires clear identification of pathogens and antibiotic susceptibility testing.

Speed Mam Color enables evidence-based therapy and sensible use of antibiotics

Speed Mam Color enables rapid infentification of the risk of antibiotic resistance

Thanks to its simple protocol, Speed Mam color can be used to develop systematic tests

When to use Speed Mam Color ?

Speed Mam Color can be used :

- To regularly test milk in particular case of mastitis outbreak in the herd

- To improve mastitis management and regular epidemiological surveillance within the herd based on the infectious profile of several milk samples

- To evaluate potential antibiotic failure of the 1st-line antibiotics and lower the risk of resistance

Quick guide

Please refer to the test instruction for more detailed procedure.

1. Inoculate the culture medium with the milk sample according to the instructions.

2. Then inoculate the gallery following the instructions for each well.

3. Place the gallery on the cardboard support and incubate at 37°C in an incubator.

Read the control wells followed by the antibiotic wells after 24h of incubation. Then read the bacterial identification wells after 48h of incubation. Please note that Mycoplasma spp. requires 7 days growth.

Speed Mam Color performances

In comparison with conventional agar medium culture :

Sensitivity : 92%
Specificity : 96% (1)

Bibliography

(1) MANNER Y. Méthodes de bactériologie des mammites cliniques, bibliographie, étude expérimentale d’un test bactériologique rapide. Thèse Vétérinaire, Nantes 2001
(2) BERTHELOT X., BERGONIER D. Diagnostic bactériologique des mammites : pourquoi, comment et qu’en attendre ? Bulletin GTV, 2001, 12, 31-33
(3) SERIEYS F., FAROULT B. : Plans de traitement des infections mammaires : diagnostic étiologique, Bulletin GTV, 2001, 12, 27-29