Total thyroxin (T4) quantitative test in dogs and cats
Dog
Cat
Diagnostic test
Speed T4 gives accurate quantitative results, directly at the patient's bedside in 15 minutes only.
Speed T4 enable veterinarians to screen for canine hypothyroidism and feline hyperthyroidism in a single test, thanks to its extended dynamic range and low sample volume requirements.
Thanks to its Speed Reader technology, Speed T4 enables precise, fully quantitative assay validated with a reference method on a laboratory analyzer.
Speed T4 makes it possible to detect and monitor disease progression and treatment efficacy during a single visit.
For veterinary use only.
Product specifications
Sample type
50µL of serum or heparinate plasma
Target species
Dogs, Cats
Handling time
2 minutes
Incubation
5 minutes
Results
10 minutes
Operating range
8 - 90 nmol/L
Storage
Long shelf of 24 months stored between +2°C à +8°C
Presentation
Box of 6 tests
Why measure Total T4 ?
Hypothyroidism and hyperthyroidism are among the most common endocrine disorders in dogs and cats respectively.
Thyroxine is a hormone synthesised by the thyroid gland. Measuring total T4 provides useful information for selecting and diagnosing thyroid diseases.
When to measure Total T4 ?
Hypothyroidism is a common endocrine disorder in dogs. On average, dogs are diagnosed at 7 years of age, but high-risk breeds (Doberman, Pinscher, Golden Retriever, etc.) can present symptomsas early as 2 years of age.
In cats, hyperthyroidism is more commonfrom the age of 10. Certain risk factors increase the likelihood of hyperthyroidism, such as canned food or sex (females are more frequently affected).
Speed T4 can therefore be added to conventional health check-ups (biochemistry, haematology) and senior check-ups, particularly in older cats.
In dogs, the T4 assay alone is generally not sufficient to diagnose hypothyroidism and must be confirmed by a TSH assay, which can be performed with Speed TSH.
Quick guide
Remove the tests, reagents and samples from the refrigerator 30 minutes before analysis so that the test material is at room temperature.
Start a ‘new test’ on the Speed Reader or from Speed Connect and follow the on-screen instructions.
For further details, please refer to the test instructions included in the Speed T4 kits.
Recommandations
Do not use EDTA or whole blood for the sample.
The serum or plasma must be used at room temperature within one hour, and can be stored separated from the red blood cell pellet in a dry tube in the refrigerator for 7 days. After this period, the sample should be frozen at -20°C.
Cool or freeze samples at room temperature before use (do not heat).
Strictly follow the steps in the protocol and the incubation time.
Follow the pipetting method indicated in the test instructions for use.
Never mix reagents, test cassettes and calibration chips from one batch to another.
Speed T4 performances
Validation of the Speed T4 test versus chemiluminescence enzyme-linked immunosorbent assay on plasma/serum samples:
Dogs (N=82)
Cats (N=60)
Linear regression
y=0,882x + 1, 789
y=0,959x + 4,092
Correlation
R2=0,91
R2=0,96
Speed T4 correlates very well with the reference chemiluminescence enzyme-linked immunosorbent assay in cats and dogs.
Bibliography
Rannnou B et al., Evaluation of a point-of-care immunoassay (speed t4 tm) in the measurement of feline and canine total thyroxine, Poster at ESVP-ESVONC Congress 2016
Feldman EC, Nelson RW, Reusch C and Scott-Moncrieff JC. Canine and Feline Endocrinology.4th Édition, 2015. Saunders Elsevier, St Louis, Missouri. ISBN: 978-1-4557 4456-5.
Ferguson DC, Testing for Hypothyroidism in dogs. Vet Clin N Am Small Animal Pract 2007;37:647-669
Koistra HS, Secretion pattern of thyroid-stimulating hormone in dogs during euthyroidism and hypothyroidism. Domest Anim Endocrinol. 2000 Jan;18(1):19-29
Graham P.A.et al,Etiopathologic findings ofcanine hypothyroidism. Vet Clin N Am Small Anim Pract 2007;37 : 617-632
Scott-Moncrieff JC, Thyroid Disorders in the Geriatric Veterinary Patient. Vet Clin North Am Small Anim Pract. 2012 ; 42:707-25
Mooney CT, Testing for Feline Hyperthyroidism and canine hypothyroidism. JVCS, Vol.1, No. 3, July 2008.
Urbanschitz, Utility of a canine TSH assay for diagnosis and monitoring of feline hyperthyroidism. Tierarztl Prax Ausg K Kleintiere Heimtiere 2022; 50(02): 93-100
Scott-Moncrieff, Comparaison of serum concentrations of thyroid stimulating hormone in healthy dogs hypothyroid dogs and euthyroid dogs with concurrent disease. JAVMA vol 212, No 3, Feb 1998
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