Test for the quantitative dosage of circulating progesterone in bitches.
Dog
Diagnostic test
Speed Progesterone enables vets to monitor the reproductive cycle in bitches with quantitative results in just 15 minutes.
Speed Progesterone enables the vet to monitore the bitch's heat cycle, in order to detect the ideal mating or insemination date as accurately as possible.
Speed Progesterone increases the chances of pregnancy by optimising the number of matings or inseminations.
Thanks to Speed Reader technology, Speed Progesterone allows fully quantitative, accurate dosing validated with a reference method on a laboratory analyser.
For veterinary use only.
Product specifications
Sample type
100 µL of serum or heparinate plasma
Target species
Bitch
Handling time
2 minutes
Incubation
/
Results
15 minutes
Operating range
1 - 20 ng/mL
Storage
Store between +2°C à +8°C until expiry date indicated on the packaging
Presentation
Box of 6 tests
Why use Progesterone ?
The reproductive cycle is highly variable in individual bitches, making it difficult to predict reproduction.
20% of bitches ovulate ‘outside’ the period between the 10th and 14th day of heat.
Regular progesterone measurements from the start of the heat period, combined with vaginal smears, enable the ovulatory peak to be detected and the mating or insemination to be programmed to give the best chance of pregnancy.
When to measure Progesterone ?
After monitoring the stages of pro-oestrus using smears, Speed Progesterone can be used to determine the optimum date for mating or insemination.
Before ovulation, the progesterone concentration generally fluctuates between 0 and 1 ng/mL, then increases progressively at the time of the LH peak to reach 5 to 10 ng/mL at the time of ovulation.
The veterinary surgeon will plan the follow-up dates according to the results of the analyses on a personalised basis.
Quick guide
Remove the tests, reagents and samples from the refrigerator 30 minutes before analysis so that the test material is at room temperature.
Start a ‘new test’ on the Speed Reader or from Speed Connect and follow the on-screen instructions.
For further details, please refer to the test instructions included in the Speed Progesterone kits.
Recommandations
Do not use EDTA or whole blood for the sample.
The serum or plasma must be used at room temperature within one hour, and can be stored separated from the red blood cell pellet in a dry tube in the refrigerator for 3 days. After this period, the sample should be frozen at -20°C.
Cool or freeze samples at room temperature before use (do not heat).
Strictly follow the steps in the protocol and the incubation time.
Follow the pipetting method indicated in the test instructions for use.
Never mix reagents, test cassettes and calibration chips from one batch to another.
Speed Progesterone performance
Validation of the Speed Progesterone test versus the electrochemiluminescence method (CLEIA) on plasma/serum samples:
Bitches (N=165)
Linear regression
y=1,023x + 0,325
Correlation
R2=0,934
Speed Progesterone correlates very well with the reference chemiluminescence enzyme-linked immunosorbent assay in bitches.
Bibliography
England GC and Concannon PW. Determination of the Optimal Breeding Time in the Bitch: Basic Considerations. In : Recent advances in small animal reproduction. International Veterinary. Information Service, Ithaca, NY, 2002. Available in www.ivis.org. Access on June 8, 2002.
Feldman EC, Nelson RW. Canine and Feline Endocrinology and Reproduction. 3rd Edition, 2004. WB Saunders, Philadelphia, PA. ISBN : 0-7216-9315-6
Hussein HA, Schuler G, Conze T, Wehrend A (2022) Comparison of three progesterone quantification methods using blood samples drawn from bitches during the periovulatory phase, Veterinary World, 15(1): 119-123.
Maenhoudt C, Claret E, Loukeri S, Santos NR (2017), Progesterone in the reproduction of the bitch: comparative results and interpretation of Speed ProgesteroneTM and chemiluminescence immunoassay. EVSSAR Poster
Lévy X, Fontbonne A. Determining the optimal time of mating in bitches: particularities. Revta Bras. Reprod. Anim. 2007 ; 31:128-134.
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